human large vessel endothelial cell basal medium medium 200  (Fisher Scientific)

Journal: Viruses

Article Title: Impact of SARS-CoV-2 Wuhan and Omicron Variant Proteins on Type I Interferon Response

doi: 10.3390/v17040569

Figure Lengend Snippet: Mechanisms of endothelial dysfunction in COVID-19. The figure illustrates the key mechanisms driving endothelial dysfunction in COVID-19 and their downstream effects on the vascular system that can lead to fatal organ damage. ( A ) Direct SARS-CoV-2 infection: The viral spike protein binds to ACE2 receptors expressed on endothelial cells, facilitating viral entry and replication, leading to cellular damage [ , , ]. ( B ) Systemic inflammation: Elevated pro-inflammatory cytokines, including IL-6, IL-1β, and TNF-α, activate endothelial cells, inducing an amplified inflammatory response [ , , , ]. ( C ) Hypercoagulable state: Endothelial injury promotes thrombin generation and platelet aggregation, resulting in the formation of thrombi and widespread vascular occlusion [ , ]. ( D ) Hypoxia: Reduced oxygen delivery due to severe respiratory distress exacerbates endothelial dysfunction, further impairing tissue oxygenation [ , ]. ( E ) Complement activation: Overactivation of the complement cascade causes endothelial damage and contributes to pro-thrombotic states through the generation of C3 and C5 convertases [ , , ].

Article Snippet: They were cultured in Human Large Vessel Endothelial Cell Basal Medium (formerly Medium 200) (Thermo Fisher Scientific) supplemented with Large Vessel Endothelial Supplement (LVES) (Thermo Fisher Scientific), 2 mM L-glutamine (Thermo Fisher Scientific), and 100 U/mL penicillin and 100 μg/mL streptomycin (Thermo Fisher Scientific).

Techniques: Infection, Amplification, Activation Assay

Journal: F1000Research

Article Title: A humanised thrombus-on-a-chip model utilising tissue-engineered arterial constructs: A method to reduce and replace mice used in thrombosis and haemostasis research.

doi: 10.12688/f1000research.158910.1

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Article Snippet: Human Large Vessel Endothelial Cell Basal Medium (previously known as Medium 200) , Fisher Scientific (Loughborough, UK) , Media for HUVEC culture Must be kept sterile.

Techniques: Sterility, Concentration Assay, Infection, Activity Assay

Journal: iScience

Article Title: Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cells

doi: 10.1016/j.isci.2023.107232

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Article Snippet: Human Large Vessel Endothelial Cell Basal Medium (Medium 200) , ThermoFisher Scientific (Gibco) (USA) , Cat# M200500.

Techniques: Produced, Recombinant, Transfection, Modification, Saline, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Blocking Assay, Staining, Membrane, Purification, Electroporation, Luciferase, Reverse Transcription, Plasmid Preparation, Software, Polymer